ELISA:

Very sensitive method for detecting proteins

ELISA (enzyme-linked immunosorbent assay) is a commonly used process for detecting single proteins; it takes advantage of the immune system's ability to produce antibodies able to bind to and thus label specific target molecules. Antibodies for ELISA tests are produced using various biological procedures.

In the ELISA test, the protein of interest (if present) binds to its respective antibody, which is fused to an inert carrier medium. This bonding triggers an enzymatic reaction resulting in the production of a coloured precipitate.

ELISA tests are now widely used in medical diagnosis and in labs, where the detection of individual proteins is needed. Note that ELISA is different from processes for detecting DNA or DNA sequences.


Glossary

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