Transplastomic plants for biocontainment (biological confinment of transgenes)

OBJECTIVES:

To assess the promise of using transplastomic plants for containing foreign genes, researchers intend to produce a document compiling all of the information that is currently available on the topic. In addition to the review, this project also has an experimental component to measure how often genetically modified chloroplasts “leak” into pollen.


APPROACH:

Review

Transplastomic sprout
Transplastomic sprout
Researchers evaluate plastid transformation for biocontainment by compiling and evaluating relevant data on important crops. Crop-specific data is compiled on:

  • the way plastids are inherited
  • the feasibility of plastid transformation
  • possibilities of combining plastid transformation with other biocontainment strategies.

Evaluation of plastid transformation as a containment strategy

To get an idea of how much "paternal leakage" occurs - that is, when some plastids end up in pollen - researchers use transplastomic plants as pollen donors to see if the resulting seeds turn out to be GMOs. To accomplish this, researchers start by generating transplastomic tobacco lines with an antibiotic resistance marker gene. Because the plants will be transplastomic, the pollen should be free of the antibiotic resistance gene.

To find out if this is really the case, male sterile tobacco plants are pollinated using pollen from the transplastomic tobacco. Because male sterile tobacco does not produce pollen, the resulting seed set will have been entirely fathered by the transplastomic tobacco. To find out of the resulting seed set is truly free of transformed plastids, the seeds are grown on antibiotics, which would normally kill the plant. If some of the seedlings survive, it would suggest that some transformed plastids were indeed transferred via pollen.


SHORT RESULTS:

Click to enlarge!
Click to enlarge!
Review

The review found that for the large part, data on the frequency of transgene transmission via plastids by pollination is scarce. Therefore, it is particularly important to conduct the research involved with this project to obtain data on this important biocontainment issue.

The review will be published in the scientific journal Topics in Current Genetics in 2007.

Evaluation of plastid transformation as containment strategy

The researchers have developed an experimental system that facilitates selection for occasional paternal plastid transmission. In a large screen, the team detected low-level paternal inheritance of transgenic plastids in tobacco.

The research led to the conclusion that plastid transformation provides an effective tool to increase the biosafety of transgenic plants. However, the method does not completely prevent transgenes in pollen and therefore must be combined with additional containment methods to eliminate the residual outcrossing risk in cases where pollen transmission must be prevented altogether.

The research has been published in the scientific journal Proceedings of the National Academy of Sciences.


More information:

Chloroplast transformation and transgene containment

Public Deliverables of the Co-Extra project



DETAILED SCIENTIFIC RESULTS:

PARTICIPANTS:

NAME / ORGANISATIONCONTACT INFORMATION
Ralph Bock
Max Planck Institute for Molecular Plant Physiology, Germany
Email: info@coextra.eu