Plant and food components break down over the course of time and because of processing. It is important to closely study the molecules used to identify GMOs to make sure that even after some degradation, they still yield accurate results. Therefore, this task aims to make an assessment of the stability of a wide range of potential target molecules for GMO testing.
The types of samples being tested for instability include, but are not limited to:
The way, samples are prepared for the lab could also be a source of biased instability effects on targeted analytes. Therefore, researchers look for biases depending on the type of tissue taken for analysis, the processing state of the sample (e.g. heated versus air dried or dry milled versus wet milled), the DNA extraction method, and the quantitative detection method used.
The number of scientific publications reporting on possible bias in GM quantity estimations using real-time PCR based methods is growing, and cannot be ignored. However, in most cases the experimental design does not permit clear conclusions to be drawn, because data produced could have several possible explanations.
GMO testing: Status report on the target analyte instability bias studies
This report describes the results of studies on target analyte instability. The purpose of the report is to assist the stakeholders in interpreting analytical results and implementing analytical procedures that may minimise the risk of bias of the resulting GMO quantity estimates.
|NAME / ORGANISATION||CONTACT INFORMATION|
|National Veterinary Institute (NVI), Norway||
|Department of Plant Genetics and Breeding (Institute for Agricultural and Fisheries Research – ILVO), Ghent, Belgium|
|GeneScan Analytics GmbH, Germany|
|Consejo Superior de Investigaciones Cientificas (CSIC-IRTA), Spain|